CD73 (C-Term) antibody

Antigen: CD73

Epitope: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388750

Additional Information

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Alternative name: CD73 (NT5E) (C-term)

Gene ID: 4907

Swiss-Prot: NP_002517

Immunogen: This CD73 (NT5E) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 528~557 amino acids from the C-terminal region of human NT5E.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: Purine 5-Prime-Nucleotidase, Ecto-5 Prime-Nucleotidase, CD73 antigen

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human NT5E.

Molecular Weight: 63367 DA

Application Details

Application Notes: The suggested dilution will be: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100 Flow cytometry

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Stem Cells, Cell Structure, Signaling, Chromatin

Restrictions: For Research Use only

Images

Western blot analysis of CD73 Pab (ABIN388750) in mouse brain tissue lysate. CD73 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Formalin-fixed and paraffin-embedded human Placenta tissue reacted with CD73 (NT5E) Antibody (C-term)(ABIN388750), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Publications

Publications: Leth-Larsen, Lund, Hansen et al.: "Metastasis-related plasma membrane proteins of human breast cancer cells identified by comparative quantitative mass spectrometry." in: Molecular & cellular proteomics : MCP, Vol. 8, Issue 6, pp. 1436-49, 2009 (PubMed).