TAU (C-Term) antibody

Antigen: TAU

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388782

Additional Information

Gene ID: 4137

UniProt: P10636

Immunogen: This TAU antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 685~714 amino acids from the C-terminal region of human TAU.

Isotype: Ig

Description: Other names: Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau, MAPT, MAPTL, MTBT1, TAU

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This TAU antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 685~714 amino acids from the C-terminal region of human TAU.

Molecular Weight: 78746 DA

Comments:

Background: The microtubule-associated protein tau (MAPT) gene transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations result in several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.

Application Details

Application Notes: The suggested dilution will be: ELISA~~1:1,000 Western blotting~~1:50~100 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Stem Cells, Cell Structure, Neurology

Restrictions: For Research Use only

Images

Western blot analysis of TAU(arrow) using rabbit polyclonal TAU Antibody (ABIN388782). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the TAU gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Kraemer, Zhang, Leverenz et al.: "Neurodegeneration and defective neurotransmission in a Caenorhabditis elegans model of tauopathy." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 17, pp. 9980-5, 2003 (PubMed).

Gamblin, Chen, Zambrano et al.: "Caspase cleavage of tau: linking amyloid and neurofibrillary tangles in Alzheimer's disease." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 17, pp. 10032-7, 2003 (PubMed).

Horiguchi, Uryu, Giasson et al.: "Nitration of tau protein is linked to neurodegeneration in tauopathies." in: The American journal of pathology, Vol. 163, Issue 3, pp. 1021-31, 2003 (PubMed).

Yoshiyama, Zhang, Bruce et al.: "Reduction of detyrosinated microtubules and Golgi fragmentation are linked to tau-induced degeneration in astrocytes." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 23, Issue 33, pp. 10662-71, 2003 (PubMed).