|+1 404 474 4654|
|+1 888 205 9894 (TF)|
POU Class 5 Homeobox 1 (POU5F1) (Glu125) antibody
|Synonyms||OCT3, OCT4, OTF3, OTF4, MGC22487, POU5F1, POU5F1L|
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), ELISA
|4 references available|
|Quantity||400 µL (0.25 mg/ml)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Alternative name||OCT4 (OCT3)|
|Immunogen||This OCT4 (OCT3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 118~148 amino acids surrounding E125 of human OCT3/4.|
|Description||Other names: POU domain class 5 transcription factor 1, POU5F1, Octamer-binding transcription factor 3, OCT-3, OTF3, OCT-4|
|Characteristics||Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This OCT4 (OCT3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 118~148 amino acids surrounding E125 of human OCT3/4.|
|Molecular Weight||38562 DA|
Background: Transcription factors containing the POU homeodomain have been shown to be important regulators of tissue-specific gene expression in lymphoid and pituitary differentiation and in early mammalian development. Two forms of OCT3 mRNA are expressed in adult tissues as a result of alternative splicing--OCT3A and OCT3B. Reverse transcriptase PCR showed low level of expression in both OCT3A and OCT3B mRNA in all adult human tissues examined. Oct3 is present in mouse oocytes before and after fertilization. When fertilized oocytes were injected with antisense Oct3 oligonucleotides or double-stranded DNA containing the octamer motif, embryonic DNA synthesis was inhibited and the embryos were arrested at the one-cell stage.
|Application Notes||The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunofluorescence~~ 1:10~50|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Stem Cells, Transcription Factors, Signaling|
|Restrictions||For Research Use only|
Takeda, Seino, Bell: "Human Oct3 gene family: cDNA sequences, alternative splicing, gene organization, chromosomal location, and expression at low levels in adult tissues." in: Nucleic acids research, Vol. 20, Issue 17, pp. 4613-20, 1992 (PubMed).
Crouau-Roy, Amadou, Bouissou et al.: "Localization of the OTF3 gene within the human MHC class I region by physical and meiotic mapping." in: Genomics, Vol. 21, Issue 1, pp. 241-3, 1994 (PubMed).
Looijenga, Stoop, de Leeuw et al.: "POU5F1 (OCT3/4) identifies cells with pluripotent potential in human germ cell tumors." in: Cancer research, Vol. 63, Issue 9, pp. 2244-50, 2003 (PubMed).
Reményi, Lins, Nissen et al.: "Crystal structure of a POU/HMG/DNA ternary complex suggests differential assembly of Oct4 and Sox2 on two enhancers." in: Genes & development, Vol. 17, Issue 16, pp. 2048-59, 2003 (PubMed).