Ubiquitin Conjugation Factor E4 B (UBE4B) (N-Term) antibody

Antigen: Ubiquitin Conjugation Factor E4 B (UBE4B)

Synonyms: E4, UFD2, HDNB1, UBOX3, KIAA0684, UFD2a, Ufd2p, AU014668, mKIAA0684, D4Bwg0973e, 4930551I19Rik, 4933406G05Rik

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388841

Additional Information

Alternative name: UBE4B

Gene ID: 10277

UniProt: O95155

Immunogen: This UBE4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 120~149 amino acids from the N-terminal region of human UBE4B.

Isotype: Ig

Description: Other names: Ubiquitin conjugation factor E4 B, Ubiquitin-fusion degradation protein 2, Homozygously deleted in neuroblastoma-1, HDNB1, KIAA0684, UFD2

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This UBE4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 120~149 amino acids from the N-terminal region of human UBE4B.

Molecular Weight: 133275 DA

Comments:

Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE4B represents an additional conjugation factor, E4, which is involved in multiubiquitin chain assembly. UBE4B also the strongest candidate among the neuroblastoma tumor suppressors.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Ubiquitin-related antibodies, Apoptosis/Necrosis, Protein Modifications, Cancer, Neurology, Cell Structure

Restrictions: For Research Use only

Images

The anti-UBE4B Pab (ABIN388841) is used in Western blot to detect UBE4B in mouse kidney tissue lysate.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Ishikawa, Nagase, Suyama et al.: "Prediction of the coding sequences of unidentified human genes. X. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro." in: DNA research : an international journal for rapid publication of reports on genes and genomes, Vol. 5, Issue 3, pp. 169-76, 1998 (PubMed).

Mahoney, Odin, White et al.: "The human homologue of the yeast polyubiquitination factor Ufd2p is cleaved by caspase 6 and granzyme B during apoptosis." in: The Biochemical journal, Vol. 361, Issue Pt 3, pp. 587-95, 2002 (PubMed).