Ubiquitin-Conjugating Enzyme E2D 1 (UBE2D1) (C-Term) antibody

Antigen: Ubiquitin-Conjugating Enzyme E2D 1 (UBE2D1)

Synonyms: SFT, UBCH5, UBC4/5, UBCH5A, E2(17)KB1, MGC28550, zgc:73096, wu:fc16h06, sft, ubch5, ubc4/5, ubch5a, e2(17)kb1, UBE2D1, MGC134214, MGC147208

Epitope: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388843

Additional Information

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Alternative name: UBE2D1 (C-term)

Gene ID: 7321

Swiss-Prot: NP_003329

Immunogen: This UBE2D1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 118~147 amino acids from the C-terminal region of human UBE2D1.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: Ubiquitin-conjugating enzyme E2-17 kDa 1, Ubiquitin-protein ligase, Ubiquitin carrier protein, E2(17)KB 1

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human UBE2D1.

Molecular Weight: 16602 DA

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Ubiquitin-related antibodies, Protein Modifications, Cancer, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-UBE2D1 Pab (ABIN388843) in mouse cerebellum tissue lysates (35ug/lane). UBE2D1 (arrow) was detected using the purified Pab.

Western blot analysis of anti-UBE2D1 Pab (ABIN388843) in, left-to-right, wild-type T3 cells (mouse mouse fibroblast cell line), wild-type T3 cells plus recombinant mouse UBE2D1(120 ng), and Hela cells. Electrophoresis performed on a 10% Bis-Tris gel. Membrane blocking with 10% milk-PBST. Blotting at 1:500 dilution of primary antibody. Data courtesy of Xiaoli Wang, MD. PhD. , Washington University School of Medicine.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.