Ubiquitin-Conjugating Enzyme E2B (UBE2B) (C-Term) antibody

Antigen: Ubiquitin-Conjugating Enzyme E2B (UBE2B)

Synonyms: HR6B, UBC2, HHR6B, RAD6B, E2-17kDa, Rad6b, mHR6B, E2-14k, 2610301N02Rik, UBE2B, fc79h10, wu:fc79h10, zgc:100921, MGC78891, hr6b, ubc2, hhr6b, rad6b, MGC89687, e2-17kda, MGC133582

Epitope: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388848

Additional Information

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Alternative name: UBE2B (C-term)

Gene ID: 7320

Swiss-Prot: NP_003328

Immunogen: This UBE2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human UBE2B.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: Ubiquitin-conjugating enzyme E2 B, Ubiquitin-protein ligase B, Ubiquitin carrier protein B, HR6B, hHR6B, E2-17 kDa

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human UBE2B.

Molecular Weight: 17312 DA

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca

Research Area: Ubiquitin-related antibodies, Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of UBE2B (arrow) using rabbit polyclonal UBE2B Antibody (E132) (ABIN388848). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the UBE2B gene.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Western blot analysis of UBE2B Antibody (C-term) (ABIN388848) in Ramos cell line lysates (35ug/lane). UBE2B (arrow) was detected using the purified Pab.