|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Chromobox Homolog 8 (CBX8) (C-Term) antibody
|Synonyms||PC3, RC1, HPC3, cbx8, MGC147589|
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), ELISA
|3 references available|
|Quantity||400 µL (0.25 mg/ml)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||This CBX8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 314~344 amino acids from the C-terminal region of human CBX8.|
|Description||Other names: Chromobox protein homolog 8, Polycomb 3 homolog, Pc3, HPC3, Rectachrome 1|
|Characteristics||Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This CBX8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 314~344 amino acids from the C-terminal region of human CBX8.|
|Molecular Weight||43437 DA|
Background: Chromobox homolog 8 (CBX8 or Pc3) is a member of Drosophila Polycomb group gene family. The polycomb group (PcG) genes are essential for maintenance of appropriate expression patterns of developmental master regulators and thus are essential for proper development. Changes in expression of PcG proteins have been associated with cancer. CBX8 is involved in maintaining the transcriptionally repressive state of genes. It modifies chromatin, rendering it heritably changed in its expressibility. Structurally, CBX4 contains 1 chromo domain, which is 40 to 50 amino acids long.
|Application Notes||The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Protein Modifications, Cancer, Signaling, Chromatin, Cell Structure|
|Restrictions||For Research Use only|
Bárdos, Saurin, Tissot et al.: "HPC3 is a new human polycomb orthologue that interacts and associates with RING1 and Bmi1 and has transcriptional repression properties." in: The Journal of biological chemistry, Vol. 275, Issue 37, pp. 28785-92, 2000 (PubMed).
Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).
Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).
|Hosts||Rabbit (34), Mouse (9), Goat (4)|
|Reactivities||Human (45), Mouse (Murine) (23), Rat (Rattus) (21), Cow (Bovine) (3), Dog (Canine) (3), Xenopus laevis (2), Zebrafish (2), Chicken (1)|
|Applications||Western Blotting (WB) (31), ELISA (15), Immunofluorescence (IF) (15), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (10), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunoprecipitation (IP) (2), Immunoelectron Microscopy (IEM) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||AA 1-260 (9), C-Term (4), N-Term (4), Center (2), Internal Region (2), Internal Region,AA 160-189 (1)|