Dystrobrevin Binding Protein 1 (DTNBP1) (N-Term) antibody

Antigen: Dystrobrevin Binding Protein 1 (DTNBP1)

Synonyms: SDY, DBND, HPS7, My031, FLJ30031, MGC20210, DKFZp564K192, sdy, AW048963, dysbindin, 5430437B18Rik, dtnbp1, MGC56462, zgc:56462, MGC83903, dbnd, hps7, my031, MGC85477, DTNBP1, MGC138010

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN389169

Additional Information

Alternative name: Dtnbp1

Gene ID: 94245

UniProt: Q91WZ8

Immunogen: This Dtnbp1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16~45 amino acids from the N-terminal region of mouse Dtnbp1.

Isotype: Ig

Description: Other names: Dysbindin, Dystrobrevin-binding protein 1, Hermansky-Pudlak syndrome 7 protein homolog, Hps7-like protein, Dtnbp1, Sdy

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This Dtnbp1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16~45 amino acids from the N-terminal region of mouse Dtnbp1.

Molecular Weight: 39651 DA

Comments:

Background: Dtnbp1 may play a role in organelle biogenesis associated with melanosomes, platelet dense granules, and lysosomes. A similar protein in mouse is a component of a protein complex termed biogenesis of lysosome-related organelles complex 1 (BLOC-1), and binds to alpha- and beta-dystrobrevins, which are components of the dystrophin-associated protein complex (DPC). Mutations are associated with Hermansky-Pudlak syndrome type 7. This protein may also be associated with schizophrenia.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Stem Cells

Restrictions: For Research Use only

Images

Western blot analysis of Dtnbpt(arrow) using rabbit polyclonal Dysbindin(Dtnbp1) Antibody (N-term) (ABIN389169). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Dtnbpt gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with Dtnbp1 Antibody (N-term) (ABIN389169), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Publications

Product: Benson, Newey, Martin-Rendon et al.: "Dysbindin, a novel coiled-coil-containing protein that interacts with the dystrobrevins in muscle and brain." in: The Journal of biological chemistry, Vol. 276, Issue 26, pp. 24232-41, 2001 (PubMed).