Cadherin 5 (CDH5) (N-Term) antibody

Antigen: Cadherin 5 (CDH5)

Synonyms: UVO, CDHE, ECAD, LCAM, Arc-1, CD324, 7B4, Vec, Cd144, VE-Cad, AA408225, VECAD2, VE-cadherin-2, Pcdh14, VE-cad-2, CDH5, wu:fc88a07

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN389186

Additional Information

Alternative name: VE Cadherin (CDH5)

Gene ID: 1003

UniProt: P33151

Immunogen: This CDH5 (N-term) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 113~142 amino acids from the N-term of human CDH5.

Isotype: Ig

Description: Other names: Cadherin-5,Vascular endothelial cadherin,VE-cadherin,7B4 antigen,CD144 antigen,CDH5

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This CDH5 (N-term) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 113~142 amino acids from the N-term of human CDH5.

Molecular Weight: 87516 DA

Comments:

Background: CDH5 is a classical cadherin from the cadherin superfamily and is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. It is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50-100 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure, Cancer

Restrictions: For Research Use only

Images

Western blot analysis of anti-CDH5 Pab (ABIN389186) in 293 cell line lysates (35ug/lane). CDH5(arrow) was detected using the purified Pab (1:60 dilution).

Flow cytometric analysis of 293 cells using VE Cadherin Antibody (CDH5) (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Breviario, Caveda, Corada et al.: "Functional properties of human vascular endothelial cadherin (7B4/cadherin-5), an endothelium-specific cadherin." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 15, Issue 8, pp. 1229-39, 1995 (PubMed).