LGR5 antibody (N-Term)

Catalog No. ABIN389215

This Rabbit Polyclonal antibody specifically detects LGR5 in WB. It exhibits reactivity toward Mouse. It has been mentioned in 4+ publications

Quick Overview for LGR5 antibody (N-Term) (ABIN389215)

Target: LGR5 (Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LGR5 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: RB14764

Product Details anti-LGR5 Antibody

Binding Specificity: AA 238-270, N-Term

Predicted Reactivity: H

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This LGR5/GPR49 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 238-270 amino acids from the N-terminal region of human LGR5/GPR49.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-LGR5 antibody (ABIN389215)

Usui, Sakurai, Enjoji, Kawasaki, Umata, Ohama, Fujiwara, Yabe, Tsuji, Yamawaki, Hazama, Takenouchi, Nakajima, Tsunedomi, Suzuki, Nagano, Sato: "Establishment of a Novel Model for Anticancer Drug Resistance in Three-Dimensional Primary Culture of Tumor Microenvironment." in: Stem cells international, Vol. 2016, pp. 7053872, (2017) (PubMed).

Fender, Nutter, Bertrand, Sigounas: "Notch-1 Promotes Stemness and Epithelial to Mesenchymal Transition in Colorectal Cancer." in: Journal of cellular biochemistry, (2015) (PubMed).

Jardé, Kass, Staples, Lescesen, Carne, Oliva, McMurrick, Abud: "ERBB3 Positively Correlates with Intestinal Stem Cell Markers but Marks a Distinct Non Proliferative Cell Population in Colorectal Cancer." in: PLoS ONE, Vol. 10, Issue 9, pp. e0138336, (2015) (PubMed).

Kanwar, Yu, Nautiyal, Patel, Majumdar: "The Wnt/beta-catenin pathway regulates growth and maintenance of colonospheres." in: Molecular cancer, Vol. 9, pp. 212, (2010) (PubMed).

Target Details for LGR5

Target: LGR5 (Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5))

Alternative Name: LGR5/GPR49

Background: LGR5/GPR49 is an orphan receptor. It may be an important receptor for signals controlling growth and differentiation of specific embryonic tissues.

Molecular Weight: 99998

Gene ID: 8549

NCBI Accession: NP_001264155, NP_003658

UniProt: O75473

Pathways: WNT Signaling