Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5) (Center) antibody

Antigen: Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5)

Synonyms: FEX, HG38, GPR49, GPR67, GRP49, MGC117008, lgr5a

Epitope: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN389219

Additional Information

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Alternative name: LGR5 (GPR49) (Center)

Gene ID: 8549

Swiss-Prot: O75473

Immunogen: This LGR5 (GPR49) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 451~480 amino acids from the center region of human LGR5/GPR49.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: Leucine-rich repeat-containing G-protein coupled receptor 5 [Precursor], Orphan G-protein coupled receptor HG38, G-protein coupled receptor 49, G-protein coupled receptor 67, LGR5, GPR49, GPR67

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human LGR5/GPR49.

Molecular Weight: 99867 DA

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca

Research Area: Stem Cells, Cell Signaling, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-LGR5/GPR49 Antibody (Center) (ABIN389219) in K562 cell line lysates (35ug/lane). LGR5/GPR49(arrow) was detected using the purified Pab (1:60dilution).

Formalin-fixed and paraffin-embedded human brain tissue reacted with LGR5/GPR49 antibody (Center) (ABIN389219), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

LGR5/GPR49 Antibody (Center) (ABIN389219) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the LGR5/GPR49 antibody detected the LGR5/GPR49 protein (arrow)(Kindly offered by Dr. Li).