Glypican 4 (GPC4) (Center) antibody

Antigen: Glypican 4 (GPC4)

Synonyms: K-glypican, AF311610, AI385680, AI661372, 9530073D23Rik, kny, gpc6, gpc4,, gpc4/6, knypek, chunp6920, wu:fc47a08, wu:fe05f10, xgly4, MGC85093, Xgly4, glypican-4

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN389289

Additional Information

Alternative name: GPC4

Gene ID: 2239

UniProt: O75487

Immunogen: This GPC4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 402~431 amino acids from the Center region of human GPC4.

Isotype: Ig

Description: Other names: Glypican-4, K-glypican, Secreted glypican-4, GPC4

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This GPC4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 402~431 amino acids from the Center region of human GPC4.

Molecular Weight: 62412 DA

Comments:

Background: Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The GPC4 gene is adjacent to the 3' end of GPC3 and may also play a role in Simpson-Golabi-Behmel syndrome.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Signaling, Stem Cells, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of GPC4 Antibody (Center) (ABIN389289) in K562 cell line lysates (35ug/lane). GPC4 (arrow) was detected using the purified Pab.

Western blot analysis of GPC4 Antibody (Center) (ABIN389289) in 293 cell line lysates (35ug/lane). GPC4 (arrow) was detected using the purified Pab.

Publications

Product: Karumanchi, Jha, Ramchandran et al.: "Cell surface glypicans are low-affinity endostatin receptors." in: Molecular cell, Vol. 7, Issue 4, pp. 811-22, 2001 (PubMed).