U2 Small Nuclear RNA Auxiliary Factor 1 (U2AF1) (Center S70) antibody

Antigen: U2 Small Nuclear RNA Auxiliary Factor 1 (U2AF1)

Synonyms: RN, FP793, U2AF35, U2AFBP, RNU2AF1, DKFZp313J1712, U2AF1, MGC151885, MGC131026

Epitope: Center S70

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN389304

Additional Information

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Alternative name: U2AF1 (Center S70)

Gene ID: 7307

Swiss-Prot: Q01081

Immunogen: This U2AF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62~91 amino acids from the Center region of human U2AF1.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: Splicing factor U2AF 35 kDa subunit, U2 auxiliary factor 35 kDa subunit, U2 snRNP auxiliary factor small subunit, U2AF1, U2AF35, U2AFBP

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the Center region of human U2AF1.

Molecular Weight: 27872 DA

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca

Research Area: Stem Cells

Restrictions: For Research Use only

Images

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with U2AF1 antibody (Center S70), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Western blot analysis of anti-U2AF1 Antibody (Center S70) (ABIN389304) in Ramos cell line lysates (35ug/lane).U2AF1(arrow) was detected using the purified Pab.

Western blot analysis of U2AF1 (arrow) using rabbit polyclonal U2AF1 Antibody (Center S70) (ABIN389304). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the U2AF1 gene.