U2 Small Nuclear RNA Auxiliary Factor 1 (U2AF1) (Center Y114) antibody

Antigen: U2 Small Nuclear RNA Auxiliary Factor 1 (U2AF1)

Synonyms: RN, FP793, U2AF35, U2AFBP, RNU2AF1, DKFZp313J1712, U2AF1, MGC151885, MGC131026

Epitope: Center Y114

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN389305

Additional Information

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Alternative name: U2AF1 (Center Y114)

Gene ID: 7307

Swiss-Prot: Q01081

Immunogen: This U2AF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 105~134 amino acids from the Center region of human U2AF1.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: Splicing factor U2AF 35 kDa subunit, U2 auxiliary factor 35 kDa subunit, U2 snRNP auxiliary factor small subunit, U2AF1, U2AF35, U2AFBP

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the Center region of human U2AF1.

Molecular Weight: 27872 DA

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:50~100 Flow cytometry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca

Research Area: Stem Cells

Restrictions: For Research Use only

Images

Western blot analysis of anti-U2AF1 Antibody (Center Y114) (ABIN389305) in Ramos cell line lysates (35ug/lane).U2AF1(arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human lung carcinoma reacted with NRP1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

U2AF1 Antibody (Center Y114) (ABIN389305) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.