24-Dehydrocholesterol Reductase (DHCR24) (Center) antibody

Antigen: 24-Dehydrocholesterol Reductase (DHCR24)

Synonyms: mKIAA0018, 2310076D10Rik, 5830417J06Rik, MGC82737, zgc:101638, MGC166411, KIAA0018, SELADIN1, Nbla03646, seladin-1

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN389353

Additional Information

Alternative name: DHCR24

Gene ID: 1718

UniProt: Q15392

Immunogen: This DHCR24 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 225~254 amino acids from the Center region of human DHCR24.

Isotype: Ig

Description: Other names: Diminuto/dwarf1 homolog, Seladin-1, 3-beta-hydroxysterol delta-24-reductase, 24-dehydrocholesterol reductase, DHCR24, KIAA0018

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This DHCR24 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 225~254 amino acids from the Center region of human DHCR24.

Molecular Weight: 60101 DA

Comments:

Background: DHCR24 is a flavin adenine dinucleotide (FAD)-dependent oxidoreductase which catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. This protein contains a leader sequence that directs it to the endoplasmic reticulum membrane. Missense mutations in DHCR24 gene have been associated with desmosterolosis. Also, reduced expression of DHCR24 gene occurs in the temporal cortex of Alzheimer disease patients and overexpression has been observed in adrenal gland cancer cells.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Stem Cells

Restrictions: For Research Use only

Images

Western blot analysis of anti-DHCR24 Antibody (Center)(ABIN389353) in HepG2 cell line lysates (35ug/lane). DHCR24 (arrow) was detected using the purified Pab.

Publications

Product: Greeve, Hermans-Borgmeyer, Brellinger et al.: "The human DIMINUTO/DWARF1 homolog seladin-1 confers resistance to Alzheimer's disease-associated neurodegeneration and oxidative stress." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 20, Issue 19, pp. 7345-52, 2000 (PubMed).

Waterham, Koster, Romeijn et al.: "Mutations in the 3beta-hydroxysterol Delta24-reductase gene cause desmosterolosis, an autosomal recessive disorder of cholesterol biosynthesis." in: American journal of human genetics, Vol. 69, Issue 4, pp. 685-94, 2001 (PubMed).