Activated Leukocyte Cell Adhesion Molecule (ALCAM) (N-Term) antibody

Antigen: Activated Leukocyte Cell Adhesion Molecule (ALCAM)

Synonyms: MEMD, CD166, FLJ38514, MGC71733, BEN, SC1, MuSC, AI853494, DM-GRASP, MGC27910, cd166, ALCAM, JC7, MGC83026, memd, MGC147089, DKFZp459H0124

Epitope: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN389410

Additional Information

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Alternative name: CD166 (N-term)

Gene ID: 214

Swiss-Prot: Q13740

Immunogen: This CD166 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 65~95 amino acids from the N-terminal region of human ALCAM.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: CD166 antigen, Activated leukocyte cell adhesion molecule, CD166, MEMD, ALCAM

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human ALCAM.

Molecular Weight: 65102 DA

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:50~100 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Stem Cells

Restrictions: For Research Use only

Images

Western blot analysis of CD166 antibody (N-term) (ABIN389410) in NCI-H460 cell line lysates (35ug/lane). CD166 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human brain tissue reacted with CD166 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

CD166 Antibody (N-term) (ABIN389410) flow cytometric analysis of NCI-H292 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.