Prolyl 4-Hydroxylase, beta Polypeptide (P4HB) (C-Term) antibody

Antigen: Prolyl 4-Hydroxylase, beta Polypeptide (P4HB)

Synonyms: DSI, GIT, PDI, PHDB, PDIA1, PO4DB, PO4HB, PROHB, ERBA2L, P4Hbeta, Thbp, ERp59, Pdia1, PDIR, LOC100328595, p55, P4HB, DKFZp469E2031

Epitope: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN389450

Additional Information

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Alternative name: P4HB (C-term)

Gene ID: 5034

Swiss-Prot: P07237

Immunogen: This P4HB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 443~472 amino acids from the C-terminal region of human P4HB.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: Protein disulfide-isomerase, PDI, Prolyl 4-hydroxylase subunit beta, Cellular thyroid hormone-binding protein, p55, PO4DB, PDIA1, PDI, ERBA2L, P4HB

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human P4HB.

Molecular Weight: 57116 DA

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:50~100 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Stem Cells

Restrictions: For Research Use only

Images

Western blot analysis of P4HB Antibody (C-term) (ABIN389450) in CEM,HepG2,A2058 cell line lysates (35ug/lane). P4HB (arrow) was detected using the purified Pab.(2ug/ml)

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with P4HB Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Flow cytometric analysis of K562 cells using P4HB Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.