Bi-phospho-ERK1/2-T202/Y204 antibody

Antigen: Bi-phospho-ERK1/2-T202/Y204

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Dot Blot (Dot), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN389990

Additional Information

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Gene ID: 5595

Swiss-Prot: P27361

Immunogen: This Bi-phospho-ERK1/2-T202/Y204 antibody is generated from rabbits immunized with a KLH conjugated synthetic bisphosphopeptide incorporating amino acid residues surrounding phosphorylated T202 and Y204 of human ERK1.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: Mitogen-activated protein kinase 3, Extracellular signal-regulated kinase 1, ERK-1, Insulin-stimulated MAP2 kinase, MAP kinase 1, MAPK 1, p44-ERK1, ERT2, p44-MAPK, Microtubule-associated protein 2 kinase, MAPK3, ERK1, PRKM3

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic bisphosphopeptide incorporating amino acid residues surrounding phosphorylated T202 and Y204 of human ERK1.

Molecular Weight: 43136 DA

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blot~~1:100~500 Dot Blot ~~ 1:500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step 2-sites phosphospecific peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Signaling, Protein Modifications, Cell Cycle, Phospho-specific antibodies, Cell Structure

Restrictions: For Research Use only

Images

Dot blot analysis of Bi-phospho-ERK1/2-T202/Y204 Antibody (ABIN389990) on nitrocellulose membrane. 50ng of Bisphospho-peptide or Non Phosphorylated peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Western blot analysis of ERK1/2 (arrow) using rabbit polyclonal P-MAPK(T202/Y204) (ABIN389990). HAOSMC cell lysates either transiently induced (Lane 2) or noninduced with the PDGF (Lane 1)(2 ug/lane).

Western blot analysis of ERK1/2 (arrow) using rabbit polyclonal P-MAPK(T202/Y204) (ABIN389990). HBEpC cell lysates either transiently induced (Lane 2) or noninduced with the EGF (Lane 1)(2 ug/lane) .