ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 7 (ABCB7) (C-Term) antibody

Antigen: ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 7 (ABCB7)

Synonyms: ABC7, ASAT, Atm1p, EST140535, Abc7, AA517758, AU019072, CG7955, dABCB7, DmelCG7955, ABCB7, GB11034, fc20c02, wu:fc20c02

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN390062

Additional Information

Alternative name: ABCB7

Gene ID: 22

UniProt: NP_004290

Immunogen: This ABCB7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 723~752 amino acids from the C-terminal region of human ABCB7.

Isotype: Ig

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This ABCB7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 723~752 amino acids from the C-terminal region of human ABCB7.

Molecular Weight: 82641 DA

Comments:

Background: The membrane-associated protein ABCB7 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance as well as antigen presentation. This gene encodes a half-transporter involved in the transport of heme from the mitochondria to the cytosol. With iron/sulfur cluster precursors as its substrates, this protein may play a role in metal homeostasis. Mutations in this gene have been implicated in X-linked sideroblastic anemia with ataxia.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure

Restrictions: For Research Use only

Images

The anti-ABCB7 C-term Pab (ABIN390062) is used in Western blot to detect ABCB7 in Jurkat cell lysate.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Savary, Allikmets, Denizot et al.: "Isolation and chromosomal mapping of a novel ATP-binding cassette transporter conserved in mouse and human." in: Genomics, Vol. 41, Issue 2, pp. 275-8, 1997 (PubMed).

Shimada, Okuno, Kawai et al.: "Cloning and chromosomal mapping of a novel ABC transporter gene (hABC7), a candidate for X-linked sideroblastic anemia with spinocerebellar ataxia." in: Journal of human genetics, Vol. 43, Issue 2, pp. 115-22, 1998 (PubMed).

Mao, Fu, Wu et al.: "Identification of genes expressed in human CD34(+) hematopoietic stem/progenitor cells by expressed sequence tags and efficient full-length cDNA cloning." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 14, pp. 8175-80, 1998 (PubMed).

Csere, Lill, Kispal: "Identification of a human mitochondrial ABC transporter, the functional orthologue of yeast Atm1p." in: FEBS letters, Vol. 441, Issue 2, pp. 266-70, 1999 (PubMed).

Allikmets, Raskind, Hutchinson et al.: "Mutation of a putative mitochondrial iron transporter gene (ABC7) in X-linked sideroblastic anemia and ataxia (XLSA/A)." in: Human molecular genetics, Vol. 8, Issue 5, pp. 743-9, 1999 (PubMed).