Gremlin (C-Term) antibody

Antigen: Gremlin

Synonyms: ld, Drm, Cktsf1b1, DRM, PIG2, DAND2, IHG-2, GREMLIN, CKTSF1B1, MGC126660, drm, GREM1, grem1, cktsf1b1, MGC136702, zgc:136702, pig2, dand2, ihg-2, gremlin

Epitope: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN390078

Additional Information

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Alternative name: GREMLIN (C-term)

Gene ID: 23892

Swiss-Prot: O70326

Immunogen: This GREMLIN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 154~184 amino acids from the C-terminus of human Gremlin.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: Cysteine KNOT superfamily 1, BMP antagonist 1

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminus of human Gremlin.

Molecular Weight: 20697 DA

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Neurology, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-Gremlin Pab (ABIN390078) in CEM cell line lysates (35ug/lane). Gremlin (arrow) was detected using the purified Pab.

Western blot analysis of anti-Gremlin Pab (ABIN390078) in mouse liver cell lysate. Gremlin (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Formalin-PBS and Bouin embedded paraffin human renal tissue pretreated with heat induced epitope retrieval Citrate buffer pH 6.0 in microwave. Dilution of primary antibody was 1:50. Detection method was ABC system or Vectastaint Elite VECTOR. Data and protocol courtesy of Dr. Maria E. Burgos of Universidad Austral de Chile.

Publications

Publications: Jara, Chacuon, Burgos et al.: "Expression of gremlin, a bone morphogenetic protein antagonist,is associated with vascular calcification in uraemia." in: Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, Vol. 24, Issue 4, pp. 1121-9, 2009 (PubMed).

Cooney, Baker, Brain et al.: "NOD2 stimulation induces autophagy in dendritic cells influencing bacterial handling and antigen presentation." in: Nature medicine, Vol. 16, Issue 1, pp. 90-7, 2010 (PubMed).