Cryptochrome 2 (Photolyase-Like) (CRY2) (C-Term) antibody

Antigen: Cryptochrome 2 (Photolyase-Like) (CRY2)

Synonyms: HCRY2, PHLL2, FLJ10332, KIAA0658, gCry2, Cry2, Cry, GB10211, AT-PHH1, CRY2, CRYPTOCHROME 2, CRYPTOCHROME 2 APOPROTEIN, F19P19.14, F19P19_14, FHA, PHH1, AV006279, D130054K12Rik, cry2, phll1, xCRY1

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN390080

Additional Information

Alternative name: Cry2

Gene ID: 1408

UniProt: NP_066940

Immunogen: This Cry2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 564~593 amino acids from the C-terminal region of human CRY2.

Isotype: Ig

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This Cry2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 564~593 amino acids from the C-terminal region of human CRY2.

Molecular Weight: 66946 DA

Comments:

Background: Various biochemical, physiological and behavioural processes display circadian rhythms controlled by an internal biological clock. The central ﾓgears?driving this clock appear to be composed of an autoregulatory transcription/posttranslation-based feedback loop. Cryptochrome 1 (CRY1) and 2 (CRY2) are DNA-binding flavoproteins that bear some homology to blue-light receptors and photolyases. In Drosophila, CRY is a photoreceptor for the circadian clock where it binds to the clock component TIM in a light-dependent fashion and blocks its function. Mammalian CRY1 and CRY2 function via light-independent interactions with circadian genes CLOCK and BMAL1, as well as with PER1, PER2, and TIM. They seem to act as light-independent components of the circadian clock and likely regulate Per1 transcriptional cycling via interactions with both the activator and its feedback inhibitors. Mutant mice not expressing the Cry1 or Cry2 protein display accelerated and delayed periodicity of locomotor activity, respectively. It appears that the combination of both proteins working together is essential to synchronize the organism to circadian phases. A critical balance between Cry1 and Cry2 is required for proper clock function, in complete darkness, double-mutant mice present with instantaneous arrhythmicity, indicating the absence of an internal circadian clock.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Translation Factors, Cell Cycle, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of hCry2-R579 (ABIN390080) in mouse liver tissue and HepG2 cell line lysates (35ug/lane). CRY2 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Hsu, Zhao, Zhao et al.: "Putative human blue-light photoreceptors hCRY1 and hCRY2 are flavoproteins." in: Biochemistry, Vol. 35, Issue 44, pp. 13871-7, 1996 (PubMed).

Kobayashi, Kanno, Smit et al.: "Characterization of photolyase/blue-light receptor homologs in mouse and human cells." in: Nucleic acids research, Vol. 26, Issue 22, pp. 5086-92, 1999 (PubMed).

van der Horst, Muijtjens, Kobayashi et al.: "Mammalian Cry1 and Cry2 are essential for maintenance of circadian rhythms." in: Nature, Vol. 398, Issue 6728, pp. 627-30, 1999 (PubMed).

Griffin, Staknis, Weitz: "Light-independent role of CRY1 and CRY2 in the mammalian circadian clock." in: Science (New York, N.Y.), Vol. 286, Issue 5440, pp. 768-71, 1999 (PubMed).

Ozgur, Sancar: "Purification and properties of human blue-light photoreceptor cryptochrome 2." in: Biochemistry, Vol. 42, Issue 10, pp. 2926-32, 2003 (PubMed).