Sphingosine-1-Phosphate Receptor 3 (S1PR3) (N-Term) antibody

Antigen: Sphingosine-1-Phosphate Receptor 3 (S1PR3)

Synonyms: si:dkeyp-31e2.1, EDG3, LPB3, S1P3, EDG-3, FLJ37523, FLJ93220, MGC71696, EDG6, LPC1, S1P4, SLP4

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN390084

Additional Information

Alternative name: EDG3

Gene ID: 1903

UniProt: Q99500

Immunogen: This EDG3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 6~36 amino acids from the N-terminal region of human EDG3.

Isotype: Ig

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This EDG3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 6~36 amino acids from the N-terminal region of human EDG3.

Molecular Weight: 42250 DA

Comments:

Background: EDG3 is a member of the EDG family of receptors, which are G protein-coupled receptors. This protein has been identified as a functional receptor for sphingosine 1-phosphate and likely contributes to the regulation of angiogenesis and vascular endothelial cell function.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Cycle, Cell Structure

Restrictions: For Research Use only

Images

The anti-EDG3 N-term Antibody (ABIN390084) is used in Western blot to detect EDG3 in mouse kidney lysate.

Publications

Product: Ancellin, Hla: "Differential pharmacological properties and signal transduction of the sphingosine 1-phosphate receptors EDG-1, EDG-3, and EDG-5." in: The Journal of biological chemistry, Vol. 274, Issue 27, pp. 18997-9002, 1999 (PubMed).

Lee, Thangada, Claffey et al.: "Vascular endothelial cell adherens junction assembly and morphogenesis induced by sphingosine-1-phosphate." in: Cell, Vol. 99, Issue 3, pp. 301-12, 1999 (PubMed).

Licht, Tsirulnikov, Reuveni et al.: "Induction of pro-angiogenic signaling by a synthetic peptide derived from the second intracellular loop of S1P3 (EDG3)." in: Blood, Vol. 102, Issue 6, pp. 2099-107, 2003 (PubMed).