Low Density Lipoprotein Receptor-Related Protein 6 (LRP6) (C-Term,Thr1546) antibody

Antigen: Low Density Lipoprotein Receptor-Related Protein 6 (LRP6)

Synonyms: ADCAD2, FLJ90062, FLJ90421, Cd, C030016K15Rik, lrp6-A, Xlrp6, lrp6-B, LRP6

Binding Site: C-Term,Thr1546

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN390100

Additional Information

Alternative name: LRP6

Gene ID: 4040

UniProt: O75581

Immunogen: This LRP6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1538~1568 amino acids from the C-terminal region of human LRP6.

Isotype: Ig

Description: Other names: LDL receptor-related protein 6

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This LRP6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1538~1568 amino acids from the C-terminal region of human LRP6.

Molecular Weight: 180442 DA

Comments:

Background: LRP6 is essential for the Wnt/beta catenin signaling pathway, probably by acting as a coreceptor together with Frizzled for Wnt. It is a specific, high-affinity receptor for DKK1 and DKK2, but not DKK3. The interaction with DKK1 blocks LRP6-mediated Wnt/beta catenin signaling via LRP6 removal via Kremen proteins-mediated endocytosis.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blot~~1:100~500 Immunohistochemistry~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Stem Cells, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of LRP6 (arrow) using rabbit polyclonal LRP6 Antibody (C-term T1546) (ABIN390100). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the LRP6 gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with LRP6 Antibody (C-term T1546)(ABIN390100), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Publications

Product: Brown, Twells, Hey et al.: "Isolation and characterization of LRP6, a novel member of the low density lipoprotein receptor gene family." in: Biochemical and biophysical research communications, Vol. 248, Issue 3, pp. 879-88, 1998 (PubMed).