Melanoma Antigen Family A, 12 (MAGEA12) (N-Term) antibody

Antigen: Melanoma Antigen Family A, 12 (MAGEA12)

Synonyms: CT1.12, MAGE12, MAGEA12

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN390104

Additional Information

Alternative name: MAGEA12

Gene ID: 4111

UniProt: NP_005358

Immunogen: This MAGEA12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human MAGEA12.

Isotype: Ig

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This MAGEA12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human MAGEA12.

Molecular Weight: 35000 DA

Comments:

Background: MAGEA12 is a member of the melanoma antigen gene (MAGE) family. The proteins of this family are tumor-specific antigens that can be recognized by autologous cytolytic T lymphocytes. This gene is expressed in various tumors and tumor cell lines from different tissue origins, but not detected in normal tissues, except testis. The function of this gene is unknown. This and other MAGE genes form a gene cluster at chromosome Xq28 region.

Application Details

Application Notes: The suggested dilution will be: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca

Research Area: Cancer, Chromatin, Signaling, Cell Structure

Restrictions: For Research Use only

Images

MAGEA12 Antibody (S96) (ABIN390104) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the MAGEA12 antibody detected the MAGEA12 protein (arrow).

MAGEA12 antibody (N-term) (ABIN390104)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MAGEA12 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Publications

Product: Ding, Beck, Keller et al.: "Cloning and analysis of MAGE-1-related genes." in: Biochemical and biophysical research communications, Vol. 202, Issue 1, pp. 549-55, 1994 (PubMed).