|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Melanoma Antigen Family A, 2 (MAGEA2) (N-Term) antibody
|Synonyms||CT1.2, MAGE2, MAGEA2A, MGC131923, Mage-a2, MGC124065, MGC151281, MAGEA2|
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), ELISA
|4 references available|
|Quantity||0.1 mg (0.25 mg/ml)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||This MAGEA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human MAGEA2.|
|Characteristics||Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This MAGEA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human MAGEA2.|
|Molecular Weight||35055 DA|
Background: MAGEA2 is a member of the MAGEA gene family. The members of this family have their entire coding sequences located in the last exon, and the encoded proteins show 50 to 80% sequence identity between each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are expressed at a high level in a number of tumors of various histologic types, and are silent in normal tissues with the exception of testis and placenta. The MAGEA genes are clustered on chromosome Xq28. They may be implicated in some hereditary disorders, such as dyskeratosis congenita. This gene has two identical copies at different loci.
|Application Notes||The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Cancer, Transcription Factors, Signaling, Chromatin, Cell Structure|
|Restrictions||For Research Use only|
van der Bruggen, Traversari, Chomez et al.: "A gene encoding an antigen recognized by cytolytic T lymphocytes on a human melanoma." in: Science (New York, N.Y.), Vol. 254, Issue 5038, pp. 1643-7, 1992 (PubMed).
De Plaen, Arden, Traversari et al.: "Structure, chromosomal localization, and expression of 12 genes of the MAGE family." in: Immunogenetics, Vol. 40, Issue 5, pp. 360-9, 1994 (PubMed).
De Smet, Lurquin, van der Bruggen et al.: "Sequence and expression pattern of the human MAGE2 gene." in: Immunogenetics, Vol. 39, Issue 2, pp. 121-9, 1994 (PubMed).
Rogner, Wilke, Steck et al.: "The melanoma antigen gene (MAGE) family is clustered in the chromosomal band Xq28." in: Genomics, Vol. 29, Issue 3, pp. 725-31, 1996 (PubMed).
|Applications||Immunofluorescence (IF) (15), Western Blotting (WB) (6), ELISA (5), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (5), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunoelectron Microscopy (IEM) (1), Immunoprecipitation (IP) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||N-Term (2), AA 96-108 (1)|