Melanoma Antigen Family A, 2 (MAGEA2) (N-Term) antibody

Antigen: Melanoma Antigen Family A, 2 (MAGEA2)

Synonyms: CT1.2, MAGE2, MAGEA2A, MGC131923, Mage-a2, MGC124065, MGC151281, MAGEA2

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN390106

Additional Information

Alternative name: MAGEA2

Gene ID: 4101

UniProt: NP_005352

Immunogen: This MAGEA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human MAGEA2.

Isotype: Ig

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This MAGEA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human MAGEA2.

Molecular Weight: 35055 DA

Comments:

Background: MAGEA2 is a member of the MAGEA gene family. The members of this family have their entire coding sequences located in the last exon, and the encoded proteins show 50 to 80% sequence identity between each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are expressed at a high level in a number of tumors of various histologic types, and are silent in normal tissues with the exception of testis and placenta. The MAGEA genes are clustered on chromosome Xq28. They may be implicated in some hereditary disorders, such as dyskeratosis congenita. This gene has two identical copies at different loci.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cancer, Transcription Factors, Signaling, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

The anti-MAGEA2 N-term Antibody (ABIN390106) is used in Western blot to detect MAGEA2 in HL60 lysate.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: van der Bruggen, Traversari, Chomez et al.: "A gene encoding an antigen recognized by cytolytic T lymphocytes on a human melanoma." in: Science (New York, N.Y.), Vol. 254, Issue 5038, pp. 1643-7, 1992 (PubMed).

De Plaen, Arden, Traversari et al.: "Structure, chromosomal localization, and expression of 12 genes of the MAGE family." in: Immunogenetics, Vol. 40, Issue 5, pp. 360-9, 1994 (PubMed).

De Smet, Lurquin, van der Bruggen et al.: "Sequence and expression pattern of the human MAGE2 gene." in: Immunogenetics, Vol. 39, Issue 2, pp. 121-9, 1994 (PubMed).

Rogner, Wilke, Steck et al.: "The melanoma antigen gene (MAGE) family is clustered in the chromosomal band Xq28." in: Genomics, Vol. 29, Issue 3, pp. 725-31, 1996 (PubMed).