Matrix Metallopeptidase 10 (Stromelysin 2) (MMP10) (C-Term) antibody

Antigen: Matrix Metallopeptidase 10 (Stromelysin 2) (MMP10)

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN390134

Additional Information

Alternative name: MMP10

Gene ID: 4319

UniProt: NP_002416

Immunogen: This MMP10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human MMP10.

Isotype: Ig

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This MMP10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human MMP10.

Molecular Weight: 54151 DA

Comments:

Background: Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. MMP10 degrades proteoglycans and fibronectin. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3.

Application Details

Application Notes: The suggested dilution will be: ELISA~~1:1,000 Western blot~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Stem Cells, Protein Modifications, Cancer, Chromatin, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of hMMP10-R409 (ABIN390134) in mouse spleen tissue lysates (35ug/lane). MMP10 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Sirum, Brinckerhoff: "Cloning of the genes for human stromelysin and stromelysin 2: differential expression in rheumatoid synovial fibroblasts." in: Biochemistry, Vol. 28, Issue 22, pp. 8691-8, 1990 (PubMed).

Nagase, Woessner: "Matrix metalloproteinases." in: The Journal of biological chemistry, Vol. 274, Issue 31, pp. 21491-4, 1999 (PubMed).