Matrix Metallopeptidase 11 (Stromelysin 3) (MMP11) (C-Term) antibody

Antigen: Matrix Metallopeptidase 11 (Stromelysin 3) (MMP11)

Synonyms: ST3, SL-3, STMY3

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN390135

Additional Information

Alternative name: MMP11

Gene ID: 4320

UniProt: NP_005931

Immunogen: This MMP11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human MMP11.

Isotype: Ig

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This MMP11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human MMP11.

Molecular Weight: 54595 DA

Comments:

Background: Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. MMP11 may play an important role in the progression of epithelial malignancies. It is specifically expressed in stromal cells of breast carcinomas.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Stem Cells, Protein Modifications, Cancer, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-MMP11 Antibody (C-term)(ABIN390135) in 293 cell line lysates (35ug/lane). MMP11(arrow) was detected using the purified Pab.

Publications

Product: Basset, Bellocq, Wolf et al.: "A novel metalloproteinase gene specifically expressed in stromal cells of breast carcinomas." in: Nature, Vol. 348, Issue 6303, pp. 699-704, 1991 (PubMed).

Anglard, Melot, Guérin et al.: "Structure and promoter characterization of the human stromelysin-3 gene." in: The Journal of biological chemistry, Vol. 270, Issue 35, pp. 20337-44, 1995 (PubMed).