Matrix Metallopeptidase 15 (Membrane-inserted) (MMP15) (N-Term) antibody

Antigen: Matrix Metallopeptidase 15 (Membrane-inserted) (MMP15)

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN390139

Additional Information

Alternative name: MMP15

Gene ID: 4324

UniProt: NP_002419

Immunogen: This MMP15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human MMP15.

Isotype: Ig

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This MMP15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human MMP15.

Molecular Weight: 75807 DA

Comments:

Background: Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, MMP15 is a member of the membrane-type MMP (MT-MMP) subfamily, each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry ~~ 1:50~100 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Stem Cells, Cell Structure, Protein Modifications, Cancer

Restrictions: For Research Use only

Images

The anti-MMP15 N-term Antibody (ABIN390139) is used in Western blot to detect MMP15 in mouse brain tissue lysate (lane 1) and HL60 cell lysate (lane 2) lysate.

Formalin-fixed and paraffin-embedded human colon carcinoma with MMP15 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Flow cytometric analysis of WiDr cells using MMP15 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Mattei, Roeckel, Olsen et al.: "Genes of the membrane-type matrix metalloproteinase (MT-MMP) gene family, MMP14, MMP15, and MMP16, localize to human chromosomes 14, 16, and 8, respectively." in: Genomics, Vol. 40, Issue 1, pp. 168-9, 1997 (PubMed).

Sato, Tanaka, Takino et al.: "Assignment of the human genes for membrane-type-1, -2, and -3 matrix metalloproteinases (MMP14, MMP15, and MMP16) to 14q12.2, 16q12.2-q21, and 8q21, respectively, by in situ hybridization." in: Genomics, Vol. 39, Issue 3, pp. 412-3, 1997 (PubMed).