Matrix Metallopeptidase 16 (Membrane-inserted) (MMP16) (N-Term) antibody

Antigen: Matrix Metallopeptidase 16 (Membrane-inserted) (MMP16)

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN390140

Additional Information

Alternative name: MMP16

Gene ID: 4325

UniProt: NP_072086

Immunogen: This MMP16 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human MMP16.

Isotype: Ig

Description: Other names: MMP16

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This MMP16 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human MMP16.

Molecular Weight: 53kd Isoform. 69521 DA

Comments:

Background: Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene for MMP16 produces two transcripts, which encode a membrane-bound form and a soluble form of the protein. Both forms of the protein activate MMP2 by cleavage. This gene was once referred to as MT-MMP2, but was renamed as MT-MMP3 or MMP16.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca

Research Area: Stem Cells, Cell Structure, Protein Modifications, Cancer

Restrictions: For Research Use only

Images

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

MMP16 Antibody (E169) (ABIN390140) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the MMP16 antibody detected the MMP16 protein (arrow).

Publications

Product: Mattei, Roeckel, Olsen et al.: "Genes of the membrane-type matrix metalloproteinase (MT-MMP) gene family, MMP14, MMP15, and MMP16, localize to human chromosomes 14, 16, and 8, respectively." in: Genomics, Vol. 40, Issue 1, pp. 168-9, 1997 (PubMed).

Sato, Tanaka, Takino et al.: "Assignment of the human genes for membrane-type-1, -2, and -3 matrix metalloproteinases (MMP14, MMP15, and MMP16) to 14q12.2, 16q12.2-q21, and 8q21, respectively, by in situ hybridization." in: Genomics, Vol. 39, Issue 3, pp. 412-3, 1997 (PubMed).

Matsumoto, Katoh, Saito et al.: "Identification of soluble type of membrane-type matrix metalloproteinase-3 formed by alternatively spliced mRNA." in: Biochimica et biophysica acta, Vol. 1354, Issue 2, pp. 159-70, 1998 (PubMed).