G Protein-Coupled Receptor 37 (GPR37) (C-Term) antibody

Antigen: G Protein-Coupled Receptor 37 (GPR37)

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN390380

Additional Information

Alternative name: Pael-R (GPR37)

Gene ID: 2861

UniProt: NP_005293

Immunogen: This GPR37 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 224~254 amino acids from the C-terminal region of human Pael-R (GPR37).

Isotype: Ig

Description: Other names: Endothelin B receptor-like protein-1, ETBR-LP-1, Parkin-associated endothelin receptor-like receptor, endothelin receptor type B-like, PAELR, G protein-coupled receptor 37, GPR37

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This GPR37 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 224~254 amino acids from the C-terminal region of human Pael-R (GPR37).

Molecular Weight: 67114 DA

Comments:

Background: Parkinson is the second most common neurodegenerative disease after Alzheimers. About 1 percent of people over the age of 65 and 3 percent of people over the age of 75 are affected by the disease. The mutation is the most common cause of Parkinson disease identified to date. The function of Park2 is not well-known, however, it may play a role in the ubiquitin-mediated proteolytic pathway. Mutations in this gene are known to cause autosomal recessive juvenile parkinsonism. Alternative splicing of this gene produces three known products of undetermined function. Panneuronal expression of Parkin substrate Pael-R causes age-dependent selective degeneration of Drosophila dopaminergic (DA) neurons, coexpression of Parkin degrades Pael-R and suppresses its toxicity.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Neurology, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-Pael-R (GPR37) Pab (ABIN390380) in mouse brain tissue lysate. Pael-R (GPR37) (arrow) was detected using the purified Pab.

Western blot analysis of anti-Pael-R (GPR37) Antibody (C-term)(ABIN390380) in K562 cell line lysates (35ug/lane). Pael-R (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Marazziti, Golini, Gallo et al.: "Cloning of GPR37, a gene located on chromosome 7 encoding a putative G-protein-coupled peptide receptor, from a human frontal brain EST library." in: Genomics, Vol. 45, Issue 1, pp. 68-77, 1997 (PubMed).

Imai, Soda, Inoue et al.: "An unfolded putative transmembrane polypeptide, which can lead to endoplasmic reticulum stress, is a substrate of Parkin." in: Cell, Vol. 105, Issue 7, pp. 891-902, 2001 (PubMed).

Yang, Nishimura, Imai et al.: "Parkin suppresses dopaminergic neuron-selective neurotoxicity induced by Pael-R in Drosophila." in: Neuron, Vol. 37, Issue 6, pp. 911-24, 2003 (PubMed).