Aminoacylase 2 (ACY2) (N-Term) antibody

Antigen: Aminoacylase 2 (ACY2)

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN390843

Additional Information

Alternative name: ASPA

Gene ID: 443

UniProt: P45381

Immunogen: This ASPA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89~118 amino acids from the N-terminal region of human ASPA.

Isotype: Ig

Description: Other names: Aspartoacylase, Aminoacylase-2, ACY-2, ASP, ACY2, ASPA

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This ASPA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89~118 amino acids from the N-terminal region of human ASPA.

Molecular Weight: 35735 DA

Comments:

Background: ASPA is an enzyme that catalyzes the conversion of N-acetyl_L-aspartic acid (NAA) to aspartate and acetate. NAA is abundant in the brain where hydrolysis by aspartoacylase is thought to help maintain white matter. This protein is an NAA scavenger in other tissues.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:50~100 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling

Restrictions: For Research Use only

Images

Western blot analysis of ASPA Antibody (N-term) (ABIN390843) in MCF7 cell line lysates (35ug/lane). ASPA (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human skeletal muscle reacted with ASPA Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Flow cytometric analysis of MDA-MB468 cells using ASPA Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Bitto, Bingman, Wesenberg et al.: "Structure of aspartoacylase, the brain enzyme impaired in Canavan disease." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 2, pp. 456-61, 2007 (PubMed).