Protein Kinase D3 (PRKD3) antibody

Antigen: Protein Kinase D3 (PRKD3)

Synonyms: EPK2, PKD3, PRKCN, PKC-NU, nPKC-NU, PRKD3

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Certificates: ISO 9001:2008

Catalog no.: ABIN391014

Additional Information

Alternative name: PKC nu

Gene ID: 23683

UniProt: O94806

Immunogen: This PKC nu antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 361~390 amino acids from the center region of human PKC nu.

Isotype: Ig

Description: Other names: Protein kinase C, nu type, nPKC-nu, Protein kinase EPK2, PKD3

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This PKC nu antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 361~390 amino acids from the center region of human PKC nu.

Molecular Weight: 100470 DA

Comments:

Background: Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. PKC nu is one of the PKC family members. This kinase can be activated rapidly by the agonists of G protein-coupled receptors. It resides in both cytoplasm and nucleus, and its nuclear accumulation is found to be dramatically enhanced in response to its activation. This kinase can also be activated after B-cell antigen receptor (BCR) engagement, which requires intact phopholipase C gamma and the involvement of other PKC family members.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100 Immunofluorescence~~1:500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cancer, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-PKCnu Pab (ABIN391014) in lysate of HL60 cells stimulated with PMA (lane A) and mouse brain tissue lysate (lane B). PKCnu (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Upper panel, western blot analysis of GFP fusion protein expression in Panc-1 cells by using an anti-GFP antibody. Lanes 1 and 5: non-transfected cells. lanes 2 and 6: GFP-PKD-transfected cells. lanes 3 and 7: GFP-PKD2-transfected cells. lanes 4 and 8: GFP-PKD3 transfected cells. Center panel, western blot analysis of GFP fusion protein expression in Panc-1 cells by using PKD3 N-term (ABIN391014) and C-term (ABIN391015) antibodies. Lower panel, indirect immunofluorescence analysis of GFP-PKD3 fusion protein expression in Panc-1 cells by using ABIN391014 and ABIN391015 antibodies. Data courtesy of Dr. Osvaldo Rey, University of California Los Angeles.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Matthews, Dayalu, Thompson et al.: "Regulation of protein kinase Cnu by the B-cell antigen receptor." in: The Journal of biological chemistry, Vol. 278, Issue 11, pp. 9086-91, 2003 (PubMed).

Rey, Yuan, Young et al.: "Protein kinase C nu/protein kinase D3 nuclear localization, catalytic activation, and intracellular redistribution in response to G protein-coupled receptor agonists." in: The Journal of biological chemistry, Vol. 278, Issue 26, pp. 23773-85, 2003 (PubMed).

Yeaman, Ayala, Wright et al.: "Protein kinase D regulates basolateral membrane protein exit from trans-Golgi network." in: Nature cell biology, Vol. 6, Issue 2, pp. 106-12, 2004 (PubMed).