|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Protein Kinase D3 (PRKD3) (C-Term) antibody
|Synonyms||EPK2, PKD3, PRKCN, PKC-NU, nPKC-NU, PRKD3|
Alternatives Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
|3 references available|
|Quantity||0.1 mg (0.25 mg/ml)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Alternative name||PKC nu|
|Immunogen||This PKC nu antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human PKC.|
|Description||Other names: Protein kinase C, nu type, nPKC-nu, Protein kinase EPK2, PKD3|
|Characteristics||Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This PKC nu antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human PKC.|
|Molecular Weight||100470 DA|
Background: Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. PKC nu is one of the PKC family members. This kinase can be activated rapidly by the agonists of G protein-coupled receptors. It resides in both cytoplasm and nucleus, and its nuclear accumulation is found to be dramatically enhanced in response to its activation. This kinase can also be activated after B-cell antigen receptor (BCR) engagement, which requires intact phopholipase C gamma and the involvement of other PKC family members.
|Application Notes||The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100 Immunofluorescence: 1:500|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cancer, Cell Structure|
|Restrictions||For Research Use only|
Matthews, Dayalu, Thompson et al.: "Regulation of protein kinase Cnu by the B-cell antigen receptor." in: The Journal of biological chemistry, Vol. 278, Issue 11, pp. 9086-91, 2003 (PubMed).
Rey, Yuan, Young et al.: "Protein kinase C nu/protein kinase D3 nuclear localization, catalytic activation, and intracellular redistribution in response to G protein-coupled receptor agonists." in: The Journal of biological chemistry, Vol. 278, Issue 26, pp. 23773-85, 2003 (PubMed).
Yeaman, Ayala, Wright et al.: "Protein kinase D regulates basolateral membrane protein exit from trans-Golgi network." in: Nature cell biology, Vol. 6, Issue 2, pp. 106-12, 2004 (PubMed).
|Hosts||Rabbit (40), Goat (1)|
|Reactivities||Human (38), Mouse (Murine) (25), Cow (Bovine) (24), Dog (Canine) (24), Rat (Rattus) (24), Chicken (19), Guinea Pig (19), Horse (Equine) (19), Pig (Porcine) (19)|
|Applications||Immunofluorescence (IF) (26), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (12), Western Blotting (WB) (12), ELISA (8), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunohistochemistry (Paraffin-embedded Sections) pro (IHC (p)+) (2), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (IHC) (1), Immunoprecipitation (IP) (1)|
|Conjugates||Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), PE,Cy5.5 (2), Alexa Flour 488 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)|
|Epitopes||pSer910 (4), C-Term (3), Internal Region (3), Center (1), N-Term (1), pTyr463 (1)|