AMPK beta (PRKAB1) (N-Term) antibody

Antigen: AMPK beta (PRKAB1)

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391054

Additional Information

Gene ID: 5564

UniProt: NP_006244

Immunogen: This PRKAB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 11~40 amino acids from the N-terminal region of human PRKAB1.

Isotype: Ig

Description: Other names: 5'-AMP-activated protein kinase, beta-1 subunit, AMPK beta-1 chain, AMPKb

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This PRKAB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 11~40 amino acids from the N-terminal region of human PRKAB1.

Molecular Weight: 30251 DA

Comments:

Background: The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Metabolism, Cell Structure

Restrictions: For Research Use only

Images

The anti-PRKAB1 Pab (ABIN391054) is used in Western blot to detect PRKAB1 in Jurkat cell lysate (Lane 1) and mouse spleen tissue lysate (Lane 2).

Western blot analysis of anti-PRKAB1 Antibody (N-term) (ABIN391054) in mouse heart lysates (35ug/lane). PRKAB1 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Landree, Hanlon, Strong et al.: "C75, a fatty acid synthase inhibitor, modulates AMP-activated protein kinase to alter neuronal energy metabolism." in: The Journal of biological chemistry, Vol. 279, Issue 5, pp. 3817-27, 2004 (PubMed).

Andersson, Filipsson, Abbott et al.: "AMP-activated protein kinase plays a role in the control of food intake." in: The Journal of biological chemistry, Vol. 279, Issue 13, pp. 12005-8, 2004 (PubMed).

Minokoshi, Alquier, Furukawa et al.: "AMP-kinase regulates food intake by responding to hormonal and nutrient signals in the hypothalamus." in: Nature, Vol. 428, Issue 6982, pp. 569-74, 2004 (PubMed).