AMPK beta2 (PRKAB2) (N-Term) antibody

Antigen: AMPK beta2 (PRKAB2)

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391055

Additional Information

Gene ID: 5565

UniProt: NP_005390

Immunogen: This PRKAB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 11~40 amino acids from the N-terminal region of human PRKAB2.

Isotype: Ig

Description: Other names: 5'-AMP-activated protein kinase, beta-2 subunit, AMPK beta-2 chain

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This PRKAB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 11~40 amino acids from the N-terminal region of human PRKAB2.

Molecular Weight: 30302 DA

Comments:

Background: The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Metabolism, Cell Structure

Restrictions: For Research Use only

Images

PRKAB2 Antibody (A18) (ABIN391055) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the PRKAB2 antibody detected the PRKAB2 protein (arrow).

PRKAB2 Antibody (A18) (ABIN391055) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the PRKAB2 antibody detected the PRKAB2 protein (arrow).

Publications

Product: Thornton, Snowden, Carling: "Identification of a novel AMP-activated protein kinase beta subunit isoform that is highly expressed in skeletal muscle." in: The Journal of biological chemistry, Vol. 273, Issue 20, pp. 12443-50, 1998 (PubMed).

Xu, Huang, Xu et al.: "Insight into hepatocellular carcinogenesis at transcriptome level by comparing gene expression profiles of hepatocellular carcinoma with those of corresponding noncancerous liver." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 26, pp. 15089-94, 2001 (PubMed).

Minokoshi, Alquier, Furukawa et al.: "AMP-kinase regulates food intake by responding to hormonal and nutrient signals in the hypothalamus." in: Nature, Vol. 428, Issue 6982, pp. 569-74, 2004 (PubMed).