Deoxycytidine Kinase (DCK) (C-Term) antibody

Antigen: Deoxycytidine Kinase (DCK)

Synonyms: dck, MGC80405, DCK, Dck, dck2, MGC174279, wu:fe11h12, wu:fj05e11, zgc:109951, wu:fc15f06, zgc:101771, MGC127248, MGC117410, MGC138632

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391125

Additional Information

Alternative name: DCK

Gene ID: 1633

UniProt: P27707

Immunogen: This DCK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 178~207 amino acids from the C-terminal region of human DCK.

Isotype: Ig

Description: Other names: Deoxycytidine kinase, dCK

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This DCK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 178~207 amino acids from the C-terminal region of human DCK.

Molecular Weight: 30518 DA

Comments:

Background: Deoxycytidine kinase is responsible for the phosphorylation of several deoxyribonucleosides and their analogs. Deficiency of this enzyme activity is associated with resistance to antiviral and anticancer chemotherapeutic agents, whereas increased enzyme activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. It is the rate limiting enzyme in the activation of many important anticancer and retroviral drugs and its activity is often decreased in cells that are resistant to cytosine arabinoside.

Application Details

Application Notes: The suggested dilution will be: ELISA~~1:1,000 Western blotting~~1:100~500 IP~~1:500~1:1000 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cancer, Cell Structure

Restrictions: For Research Use only

Images

The anti-DCK Pab (ABIN391125) is used in Western blot to detect DCK in mouse intestine tissue lysate.

Deoxycytidine kinase(DCK) immunoprecipitated from H460cells with 7.5ug (microgram) of the dCK antibody (ABIN391125) using the Pierce classic mammalian IP kit (#45217) reagent as described as manufacturer instructions (lane 1, 3) and Current Protocols in Cell Biology, 1998, 7.2.1-7.2.21. Proteins separated on a 12% SDS gel, transferred to a PVDF membrane and probed with 1:700 dilution of DCK antibody (ABIN391125). Bands were detected using enhanced chemiluminescence (SuperSignal West Pico Chemiluminescent Substrate Kit). No specific reagents were employed to remove IgG from immunoprecipitated sample. Data courtesy of Dr. Stacy Shord, University of Illinois, Chicago.

DCK Antibody (C-term) (ABIN391125)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DCK Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Publications

Hartford, Duan, Delaney et al.: "Population-specific genetic variants important in susceptibility to cytarabine arabinoside cytotoxicity." in: Blood, Vol. 113, Issue 10, pp. 2145-53, 2009 (PubMed).

Product: Chottiner, Shewach, Datta et al.: "Cloning and expression of human deoxycytidine kinase cDNA." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 4, pp. 1531-5, 1991 (PubMed).