NIMA-Related Kinase 8 (NEK8) (N-Term) antibody

Antigen: NIMA-Related Kinase 8 (NEK8)

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391136

Additional Information

Alternative name: NEK8

Gene ID: 284086

UniProt: Q86SG6

Immunogen: This NEK8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 73~103 amino acids from the N-terminal region of human NEK8.

Isotype: Ig

Description: Other names: Serine/threonine-protein kinase Nek8, NimA-related protein kinase 8, NIMA-related kinase 12a, NEK12A

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This NEK8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 73~103 amino acids from the N-terminal region of human NEK8.

Molecular Weight: 74806 DA

Comments:

Background: NEK8 is a member of the serine/threionine protein kinase family related to NIMA (never in mitosis, gene A) of Aspergillus nidulans. The encoded protein may play a role in cell cycle progression from G2 to M phase. Mutations in the related mouse gene are associated with a disease phenotype that closely parallels the juvenile autosomal recessive form of polycystic kidney disease in humans.

Application Details

Application Notes: The suggested dilution will be: ELISA~~1:1,000 Western blotting~~1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Cycle, Cell Structure

Restrictions: For Research Use only

Images

The anti-NEK8 Pab (ABIN391136) is used in Western blot to detect NEK8 in A2058 cell lysate.

Publications

Product: Holland, Milne, Garka et al.: "Purification, cloning, and characterization of Nek8, a novel NIMA-related kinase, and its candidate substrate Bicd2." in: The Journal of biological chemistry, Vol. 277, Issue 18, pp. 16229-40, 2002 (PubMed).

Valkova, Yunis, Mak et al.: "Nek8 mutation causes overexpression of galectin-1, sorcin, and vimentin and accumulation of the major urinary protein in renal cysts of jck mice." in: Molecular & cellular proteomics : MCP, Vol. 4, Issue 7, pp. 1009-18, 2005 (PubMed).