Cyclin-Dependent Kinase-Like 5 (CDKL5) (C-Term) antibody

Antigen: Cyclin-Dependent Kinase-Like 5 (CDKL5)

Synonyms: ISSX, STK9, Stk9, BC038161, CDKL5, issx, stk9

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391362

Additional Information

Alternative name: CDKL5 (STK9)

Gene ID: 6792

UniProt: O76039

Immunogen: This CDKL5 (STK9) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 990~1019 amino acids from the C-terminal region of human STK9.

Isotype: Ig

Description: Other names: Serine/threonine protein kinase 9, Cyclin-dependent kinase-like 5

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This CDKL5 (STK9) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 990~1019 amino acids from the C-terminal region of human STK9.

Molecular Weight: 115537 DA

Comments:

Background: Defects in STK9, a dual-specificity serine/threonine kinase, are a cause of atypical Rett syndrome. Rett syndrome is an X-linked dominant disease. It is a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. Patients appear to develop normally until 6 to 18 months of age, then gradually lose speech and purposeful hand movements and develop microcephaly, seizures, autism, ataxia, intermittent hyperventilation, and stereotypic hand movements. After initial regression, the condition stabilizes and patients usually survive into adulthood. Rett syndrome due to CDKL5-associated mutations is characterized by a severe early-onset phenotype and atypical features such as infantile spasms.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Neurology, Cell Structure

Restrictions: For Research Use only

Images

The anti-STK9 Pab (ABIN391362) is used in Western blot to detect STK9 in mouse lung tissue lysate.

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with STK9 Antibody (C-term) (ABIN391362), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Publications

Product: Montini, Andolfi, Caruso et al.: "Identification and characterization of a novel serine-threonine kinase gene from the Xp22 region." in: Genomics, Vol. 51, Issue 3, pp. 427-33, 1998 (PubMed).