BAR2 (Ser261) antibody

Antigen: BAR2

Binding Site: Ser261

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391397

Additional Information

Gene ID: 154

UniProt: NP_000015

Immunogen: This BAR2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 243~272 amino acids surrounding S261 of human BAR2.

Isotype: Ig

Description: Other names: Beta-2 adrenoceptor, Beta-2 adrenoreceptor, ADRB2, ADRB2R, B2AR

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This BAR2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 243~272 amino acids surrounding S261 of human BAR2.

Molecular Weight: 46426 DA

Comments:

Background: Beta-2-adrenergic receptor is a member of the G protein-coupled receptor superfamily. This receptor is directly associated with one of its ultimate effectors, the class C L-type calcium channel Ca(V)1.2. This receptor-channel complex also contains a G protein, an adenylyl cyclase, cAMP-dependent kinase, and the counterbalancing phosphatase, PP2A. The assembly of the signaling complex provides a mechanism that ensures specific and rapid signaling by this G protein-coupled receptor.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-BAR2 Antibody (S261) (ABIN391397) in mouse kidney lysates (35ug/lane). BAR2 (arrow) was detected using the purified Pab (1:60 dilution).

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with BAR2 Antibody (S261) (ABIN391397), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Publications

Product: Cherezov, Rosenbaum, Hanson et al.: "High-resolution crystal structure of an engineered human beta2-adrenergic G protein-coupled receptor." in: Science (New York, N.Y.), Vol. 318, Issue 5854, pp. 1258-65, 2007 (PubMed).