Kinesin Family Member 3C (KIF3C) (C-Term) antibody

Antigen: Kinesin Family Member 3C (KIF3C)

Synonyms: KIF3C, zgc:110160, MGC139476, DKFZp459A044

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391675

Additional Information

Alternative name: KIF3C

Gene ID: 3797

UniProt: O14782

Immunogen: This KIF3C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 720~749 amino acids from the C-terminal region of human KIF3C.

Isotype: Ig

Description: Other names: Kinesin-like protein KIF3C , KIF3C

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This KIF3C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 720~749 amino acids from the C-terminal region of human KIF3C.

Molecular Weight: 89454 DA

Comments:

Background: KIF3C is a microtubule-based anterograde translocator for membranous organelles.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Flow cytometric~~1:10~50 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of KIF3C antibody (C-term) (ABIN391675) in Y79 cell line lysates (35ug/lane). HGK (arrow) was detected using the purified Pab.

Flow cytometric analysis of NCI-H292 cells using KIF3C Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

KIF3C Antibody (C-term) (ABIN391675) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the KIF3C Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Publications

Product: Telford, Wightman, Leek et al.: "cDNA cloning, genomic organization, and chromosomal localization of a novel human gene that encodes a kinesin-related protein highly similar to mouse Kif3C." in: Biochemical and biophysical research communications, Vol. 242, Issue 2, pp. 407-12, 1998 (PubMed).

Sardella, Navone, Rocchi et al.: "KIF3C, a novel member of the kinesin superfamily: sequence, expression, and mapping to human chromosome 2 at 2p23." in: Genomics, Vol. 47, Issue 3, pp. 405-8, 1998 (PubMed).