Nemo-Like Kinase (NLK) (C-Term) antibody

Antigen: Nemo-Like Kinase (NLK)

Synonyms: FLJ21033, DKFZp761G1211, AI194375, xnlk, nlk, Nlk.2, nlkb, zgc:111875, Xnlk, nlk2, MGC81364, NLK, nemo-like

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391786

Additional Information

Alternative name: NLK

Gene ID: 51701

UniProt: NP_057315

Immunogen: This NLK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 402~432 amino acids from the C-terminal region of human NLK.

Isotype: Ig

Description: Other names: nemo-like kinase

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This NLK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 402~432 amino acids from the C-terminal region of human NLK.

Molecular Weight: 57048 DA

Comments:

Background: Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Stem Cells, Cell Signaling, Protein Modifications, Cell Structure, Cell Cycle, Transcription Factors, Signaling, Metabolism

Restrictions: For Research Use only

Images

Western blot analysis of anti-NLK Pab (ABIN391786) in A375 cell lysate. NLK (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Brott, Pinsky, Erikson: "Nlk is a murine protein kinase related to Erk/MAP kinases and localized in the nucleus." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 3, pp. 963-8, 1998 (PubMed).

Kehrer-Sawatzki, Moschgath, Maier et al.: "Characterization of the Fugu rubripes NLK and FN5 genes flanking the NF1 (Neurofibromatosis type 1) gene in the 5' direction and mapping of the human counterparts." in: Gene, Vol. 251, Issue 1, pp. 63-71, 2000 (PubMed).

Ishitani, Kishida, Hyodo-Miura et al.: "The TAK1-NLK mitogen-activated protein kinase cascade functions in the Wnt-5a/Ca(2+) pathway to antagonize Wnt/beta-catenin signaling." in: Molecular and cellular biology, Vol. 23, Issue 1, pp. 131-9, 2002 (PubMed).

Ishitani, Ninomiya-Tsuji, Matsumoto: "Regulation of lymphoid enhancer factor 1/T-cell factor by mitogen-activated protein kinase-related Nemo-like kinase-dependent phosphorylation in Wnt/beta-catenin signaling." in: Molecular and cellular biology, Vol. 23, Issue 4, pp. 1379-89, 2003 (PubMed).

Yasuda, Tsuchiya, Yamada et al.: "Nemo-like kinase induces apoptosis in DLD-1 human colon cancer cells." in: Biochemical and biophysical research communications, Vol. 308, Issue 2, pp. 227-33, 2003 (PubMed).