Acetyl-CoA Acetyltransferase 1 (ACAT1) (C-Term) antibody

Antigen: Acetyl-CoA Acetyltransferase 1 (ACAT1)

Synonyms: T2, MAT, ACAT, THIL, SOAT, STAT, ACACT, ACAT1, RP11-215I23.2, ald, Acact, ACAT-1, AW550831, 8430426K15Rik, RATACAL, Acat-1, Acat, 6330585C21Rik, fd16h07, fd20g06, zgc:86832, wu:fd16h07, wu:fd20g06, MGC137917, MGC107795, acat1, CEH, TGH, CES2, HMSE, SES1, HMSE1, PCE-1, MGC117365, Es2, MGC109055, LOC100009551

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391807

Additional Information

Alternative name: ACAT1

Gene ID: 38

UniProt: P24752

Immunogen: This ACAT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 305~335 amino acids from the C-terminal region of human ACAT1.

Isotype: Ig

Description: Other names: Acetyl-CoA acetyltransferase, mitochondrial [Precursor], Acetoacetyl-CoA thiolase, T2,

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This ACAT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 305~335 amino acids from the C-terminal region of human ACAT1.

Molecular Weight: 45200 DA

Comments:

Background: ACAT1 is a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in the gene encoding ACAT1 are associated with the alpha-methylacetoaceticaciduria disorder,an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone.

Synonyms: T2, MAT, ACAT, THIL, SOAT, STAT, ACACT, ACAT1, RP11-215I23.2, ald, Acact, ACAT-1, AW550831, 8430426K15Rik, RATACAL, Acat-1, Acat, 6330585C21Rik, fd16h07, fd20g06, zgc:86832, wu:fd16h07, wu:fd20g06, MGC137917, MGC107795, acat1, CEH, TGH, CES2, HMSE, SES1, HMSE1, PCE-1, MGC117365, Es2, MGC109055, LOC100009551

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-ACAT1 Pab (ABIN391807) in SK-BR-3 cell line lysates (35ug/lane).ACAT1 (arrow) was detected using the purified Pab (1:60 dilution).

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ACAT1 antibody (C-term) (ABIN391807), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Publications

Product: Haapalainen, Meriläinen, Pirilä et al.: "Crystallographic and kinetic studies of human mitochondrial acetoacetyl-CoA thiolase: the importance of potassium and chloride ions for its structure and function." in: Biochemistry, Vol. 46, Issue 14, pp. 4305-21, 2007 (PubMed).

Guo, Chang, Chang: "Functionality of the seventh and eighth transmembrane domains of acyl-coenzyme A:cholesterol acyltransferase 1." in: Biochemistry, Vol. 46, Issue 35, pp. 10063-71, 2007 (PubMed).