Histidyl TRNA Synthetase (HARS) (N-Term) antibody

Antigen: Histidyl TRNA Synthetase (HARS)

Synonyms: HRS, FLJ20491

Epitope: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391819

Additional Information

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Alternative name: HARS (N-term)

Gene ID: 3035

Swiss-Prot: P12081

Immunogen: This HARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 56~86 amino acids from the N-terminal region of human HARS.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: Histidyl-tRNA synthetase, cytoplasmic, Histidine--tRNA ligase, HisRS, HARS, HRS

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human HARS.

Molecular Weight: 57411 DA

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Translation Factors, Signaling

Restrictions: For Research Use only

Images

Western blot analysis of anti-HARS Pab (ABIN391819) in K562 cell line lysates (35ug/lane).HARS (arrow) was detected using the purified Pab (1:60 dilution).

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with HARS antibody (N-term) (ABIN391819), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.