Cullin 4A (CUL4A) (N-Term) antibody

Antigen: Cullin 4A (CUL4A)

Synonyms: AW495282, MGC36573, 2810470J21Rik, RGD1563853, MGC64071, zgc:64071, CUL4A

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391852

Additional Information

Gene ID: 8451

UniProt: Q13619

Immunogen: This Cullin 4A (CUL4A) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9~39 amino acids from the N-terminal region of human CUL4a.

Isotype: Ig

Description: Other names: Cullin-4A, CUL-4A, CUL4A

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This Cullin 4A (CUL4A) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9~39 amino acids from the N-terminal region of human CUL4a.

Molecular Weight: 87680 DA

Comments:

Background: CUL4a is a core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. DCX(DET1-COP1) directs ubiquitination of JUN. DCX(DDB2) directs ubiquitination of XPC. In association with RBX1, DDB1 and DDB2 is required for histone H3 and histone H4 ubiquitination in response to ultraviolet and may be important for subsequent DNA repair. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitiantion of TP53 in response to radiation-induced DNA damage and during DNA replication. In association with DDB1 and SKP2 probably is involved in ubiquitination of CDKN1B/p27kip. Is involved in ubiquitination of HOXA9.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Ubiquitin-related antibodies, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of CUL4a (arrow) using rabbit polyclonal CUL4a Antibody (Human N-term) (ABIN391852).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CUL4a gene (Lane 2) (Origene Technologies).

Publications

Product: Wen, Duus, Friedrich et al.: "The HIV1 protein Vpr acts to promote G2 cell cycle arrest by engaging a DDB1 and Cullin4A-containing ubiquitin ligase complex using VprBP/DCAF1 as an adaptor." in: The Journal of biological chemistry, Vol. 282, Issue 37, pp. 27046-57, 2007 (PubMed).

Huang, Chen: "VprBP targets Merlin to the Roc1-Cul4A-DDB1 E3 ligase complex for degradation." in: Oncogene, Vol. 27, Issue 29, pp. 4056-64, 2008 (PubMed).

Hu, Zacharek, He et al.: "WD40 protein FBW5 promotes ubiquitination of tumor suppressor TSC2 by DDB1-CUL4-ROC1 ligase." in: Genes & development, Vol. 22, Issue 7, pp. 866-71, 2008 (PubMed).