Sestrin 1 (SESN1) (C-Term) antibody

Antigen: Sestrin 1 (SESN1)

Synonyms: zgc:91970, SESN1, pa26, sest1, DKFZp459D161

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391992

Additional Information

Alternative name: Sestrin-1

Gene ID: 27244

UniProt: Q9Y6P5

Immunogen: This Sestrin-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 319~349 amino acids from the C-terminal region of human Sestrin-1.

Isotype: Ig

Description: Other names: Sestrin-1, p53-regulated protein PA26, SEST1, PA26, SESN1

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This Sestrin-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 319~349 amino acids from the C-terminal region of human Sestrin-1.

Molecular Weight: 56557 DA

Comments:

Background: Sestrin-1 is involved in the reduction of peroxiredoxins. This protein may also be regulator of cellular growth.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca

Research Area: Cell Cycle, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of Sestrin-1 antibody (C-term) (ABIN391992) in mouse heart tissue lysates (35ug/lane). Sestrin-1 (arrow) was detected using the purified Pab.

Sestrin-1 Antibody (C-term) (ABIN391992) western blot analysis in NCI-H460,HL-60 cell line lysates (35ug/lane).This demonstrates the Sestrin-1 antibody detected the Sestrin-1 protein (arrow).

Sestrin-1 Antibody (C-term) (ABIN391992) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Velasco-Miguel, Buckbinder, Jean et al.: "PA26, a novel target of the p53 tumor suppressor and member of the GADD family of DNA damage and growth arrest inducible genes." in: Oncogene, Vol. 18, Issue 1, pp. 127-37, 1999 (PubMed).

Peeters, Debeer, Bairoch et al.: "PA26 is a candidate gene for heterotaxia in humans: identification of a novel PA26-related gene family in human and mouse." in: Human genetics, Vol. 112, Issue 5-6, pp. 573-80, 2003 (PubMed).

Budanov, Sablina, Feinstein et al.: "Regeneration of peroxiredoxins by p53-regulated sestrins, homologs of bacterial AhpD." in: Science (New York, N.Y.), Vol. 304, Issue 5670, pp. 596-600, 2004 (PubMed).