seryl-tRNA Synthetase 2, Mitochondrial (SARS2) (Center) antibody

Antigen: seryl-tRNA Synthetase 2, Mitochondrial (SARS2)

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN392301

Additional Information

Alternative name: SARS2

Gene ID: 54938

UniProt: Q9NP81

Immunogen: This SARS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 322~352 amino acids from the center region of human SARS2.

Isotype: Ig

Description: Other names: Seryl-tRNA synthetase, mitochondrial [Precursor], EC 6.1.1.11, Seryl-tRNA(Ser/Sec) synthetase, Serine--tRNA ligase, SerRSmt, SARS2, SARSM

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This SARS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 322~352 amino acids from the center region of human SARS2.

Molecular Weight: 58283 DA

Comments:

Background: SARS2 catalyzes the attachment of serine to tRNA(Ser). It is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec).

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blot~~1:50~100 Immunohistochemistry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Metabolism, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of SARS2 Antibody (Center) (ABIN392301) in Hela cell line lysates (35ug/lane). SARS2 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with SARS2 antibody (Center) (ABIN392301), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Publications

Product: Yokogawa, Shimada, Takeuchi et al.: "Characterization and tRNA recognition of mammalian mitochondrial seryl-tRNA synthetase." in: The Journal of biological chemistry, Vol. 275, Issue 26, pp. 19913-20, 2000 (PubMed).

Gibbons, Yan, Li et al.: "Genomic organization, expression, and subcellular localization of mouse mitochondrial seryl-tRNA synthetase." in: Biochemical and biophysical research communications, Vol. 317, Issue 3, pp. 774-8, 2004 (PubMed).

Müller, Deschauer, Neudecker et al.: "Late-onset mitochondrial myopathy with dystrophic changes due to a G7497A mutation in the mitochondrial tRNA(Ser(UCN)) gene." in: Acta neuropathologica, Vol. 110, Issue 4, pp. 426-30, 2005 (PubMed).