PI4K2A antibody (N-Term)

Catalog No. ABIN392587

This Rabbit Polyclonal antibody specifically detects PI4K2A in WB, IF and IHC (p). It exhibits reactivity toward Human.

Quick Overview for PI4K2A antibody (N-Term) (ABIN392587)

Target: PI4K2A (Phosphatidylinositol 4-Kinase Type 2 alpha (PI4K2A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PI4K2A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB1679

Product Details anti-PI4K2A Antibody

Binding Specificity: AA 1-30, N-Term

Purification: This antibody is purified through a protein G column, followed by dialysis against PBS.

Immunogen: This PI4K2A (PI4K II) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human PI4K2A (PI4K II).

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:10~50. WB: 1:8000. WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified monoclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

Target Details for PI4K2A

Target: PI4K2A (Phosphatidylinositol 4-Kinase Type 2 alpha (PI4K2A))

Alternative Name: PI4K2A (PI4K II)

Background: Phosphatidylinositolpolyphosphates (PtdInsPs) are centrally involved in many biologic processes, ranging from cell growth and organization of the actin cytoskeleton to endo- and exocytosis. PI4KII phosphorylates PtdIns at the D-4 position, an essential step in the biosynthesis of PtdInsPs. PI4K II is activated by detergent and inhibited by adenosine. Overexpression of PI4KII in COS-7 cells increases synthesis of PtdIns4P. Some cells overexpressing PI4KII have scattered or no perinuclear Golgi. Knockdown of PI4KII by RNA interference (RNAi) does not disrupt the Golgi, and some cells show expanded Golgi. RNAi reduces the Golgi level of PtdIns4P and blocks the association between AP1 and the trans-Golgi network. PI4KII RNAi had little effect on intra-Golgi trafficking, but it inhibited export to plasma membrane export by 35 % . It has been proposed that PI4KII generates PtdIns4P-rich domains within the Golgi that specify docking of the AP1 coat machinery.

Molecular Weight: 54022

Gene ID: 55361

NCBI Accession: NP_060895

UniProt: Q9BTU6

Pathways: Inositol Metabolic Process, Synaptic Membrane