Guanine Nucleotide Binding Protein (G Protein), beta Polypeptide 2-Like 1 (GNB2L1) (N-Term) antibody

Antigen: Guanine Nucleotide Binding Protein (G Protein), beta Polypeptide 2-Like 1 (GNB2L1)

Synonyms: MGC53289, C12-3, GNB2L1

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN392636

Additional Information

Alternative name: GNB2L1

Gene ID: 10399

UniProt: NP_006089

Immunogen: This GNB2L1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 36~66 amino acids from the N-terminal region of human GNB2L1.

Isotype: Ig

Description: Other names: Guanine nucleotide-binding protein beta subunit-like protein, P205, Receptor of activated protein kinase C 1, RACK1, Receptor for activated C kinase

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This GNB2L1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 36~66 amino acids from the N-terminal region of human GNB2L1.

Molecular Weight: 35076 DA

Comments:

Background: Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure, Cell Cycle, Transcription Factors, Signaling, Metabolism

Restrictions: For Research Use only

Images

The anti-GNB2L1 Pab (ABIN392636) is used in Western blot to detect GNB2L1 in Ramos cell lysate.

Western blot analysis of GNB2L1 (arrow) using GNB2L1 Antibody (N-term) (ABIN392636). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GNB2L1 gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Liedtke, Yun, Kyle et al.: "Protein kinase C epsilon-dependent regulation of cystic fibrosis transmembrane regulator involves binding to a receptor for activated C kinase (RACK1) and RACK1 binding to Na+/H+ exchange regulatory factor." in: The Journal of biological chemistry, Vol. 277, Issue 25, pp. 22925-33, 2002 (PubMed).

Kiely, Sant, OConnor: "RACK1 is an insulin-like growth factor 1 (IGF-1) receptor-interacting protein that can regulate IGF-1-mediated Akt activation and protection from cell death." in: The Journal of biological chemistry, Vol. 277, Issue 25, pp. 22581-9, 2002 (PubMed).

Tcherkasowa, Adam-Klages, Kruse et al.: "Interaction with factor associated with neutral sphingomyelinase activation, a WD motif-containing protein, identifies receptor for activated C-kinase 1 as a novel component of the signaling pathways of the p55 TNF receptor." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 169, Issue 9, pp. 5161-70, 2002 (PubMed).

Usacheva, Tian, Sandoval et al.: "The WD motif-containing protein RACK-1 functions as a scaffold protein within the type I IFN receptor-signaling complex." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 6, pp. 2989-94, 2003 (PubMed).

Ceci, Gaviraghi, Gorrini et al.: "Release of eIF6 (p27BBP) from the 60S subunit allows 80S ribosome assembly." in: Nature, Vol. 426, Issue 6966, pp. 579-84, 2003 (PubMed).