|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Guanine Nucleotide Binding Protein (G Protein), beta Polypeptide 2-Like 1 (GNB2L1) (N-Term) antibody
|Synonyms||MGC53289, C12-3, GNB2L1|
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), ELISA
|5 references available|
|Quantity||0.1 mg (0.25 mg/ml)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||This GNB2L1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 36~66 amino acids from the N-terminal region of human GNB2L1.|
|Description||Other names: Guanine nucleotide-binding protein beta subunit-like protein, P205, Receptor of activated protein kinase C 1, RACK1, Receptor for activated C kinase|
|Characteristics||Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This GNB2L1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 36~66 amino acids from the N-terminal region of human GNB2L1.|
|Molecular Weight||35076 DA|
Background: Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.
|Application Notes||The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure, Cell Cycle, Transcription Factors, Signaling, Metabolism|
|Restrictions||For Research Use only|
Liedtke, Yun, Kyle et al.: "Protein kinase C epsilon-dependent regulation of cystic fibrosis transmembrane regulator involves binding to a receptor for activated C kinase (RACK1) and RACK1 binding to Na+/H+ exchange regulatory factor." in: The Journal of biological chemistry, Vol. 277, Issue 25, pp. 22925-33, 2002 (PubMed).
Kiely, Sant, OConnor: "RACK1 is an insulin-like growth factor 1 (IGF-1) receptor-interacting protein that can regulate IGF-1-mediated Akt activation and protection from cell death." in: The Journal of biological chemistry, Vol. 277, Issue 25, pp. 22581-9, 2002 (PubMed).
Tcherkasowa, Adam-Klages, Kruse et al.: "Interaction with factor associated with neutral sphingomyelinase activation, a WD motif-containing protein, identifies receptor for activated C-kinase 1 as a novel component of the signaling pathways of the p55 TNF receptor." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 169, Issue 9, pp. 5161-70, 2002 (PubMed).
Usacheva, Tian, Sandoval et al.: "The WD motif-containing protein RACK-1 functions as a scaffold protein within the type I IFN receptor-signaling complex." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 6, pp. 2989-94, 2003 (PubMed).
Ceci, Gaviraghi, Gorrini et al.: "Release of eIF6 (p27BBP) from the 60S subunit allows 80S ribosome assembly." in: Nature, Vol. 426, Issue 6966, pp. 579-84, 2003 (PubMed).
|Hosts||Rabbit (44), Goat (1), Mouse (1)|
|Reactivities||Human (42), Mouse (Murine) (31), Rat (Rattus) (29), Chicken (22), Cow (Bovine) (21), Dog (Canine) (21), Sheep (Ovine) (19), Pig (Porcine) (3), Rabbit (3), Cat (Feline) (2), Xenopus laevis (2), Zebrafish (2)|
|Applications||Western Blotting (WB) (24), Immunofluorescence (IF) (17), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (13), ELISA (10), Immunohistochemistry (IHC) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Flow Cytometry (FACS) (2), Immunoprecipitation (IP) (2), Immunoelectron Microscopy (IEM) (1)|
|Conjugates||Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||N-Term (4), C-Term (2), Internal Region (1)|