Uridine-Cytidine Kinase (UCK) (C-Term) antibody

Antigen: Uridine-Cytidine Kinase (UCK)

Synonyms: CMK, UMK, CMPK, UMPK, UMP-CMPK, RP11-511I2.1, Cmpk, UMP-CMPk, 0610011D08Rik, RP23-295C20.4, MGC112719, RGD1310116, cmpk1, cmpk, MGC64474, fb52d08, fk47a07, zgc:56452, wu:fb52d08, wu:fk47a07, CMPK1, MGC137127, LOC733534, DEZ, ChemR23, CHEMERINR, MGC126105, MGC126106, V28, CCRL1, GPR13, CMKDR1, GPRV28, CMKBRL1, PPR1, CCBP2, CCR10, CCR11, VSHK1, CKR-11, CCX-CKR, CC-CKR-11, Cmkbrl1, CCX-CKR1, A630091E18Rik

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN392731

Additional Information

Alternative name: UCK

Gene ID: 51727

UniProt: P30085

Immunogen: This UCK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 140~169 amino acids from the C-terminal region of human UCK.

Isotype: Ig

Description: Other names: UMP-CMP kinase, Cytidylate kinase, Deoxycytidylate kinase, Cytidine monophosphate kinase

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This UCK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 140~169 amino acids from the C-terminal region of human UCK.

Molecular Weight: 22222 DA

Comments:

Background: UCK1 (Uridine-cytidine kinase 1) phosphorylates uridine and cytidine to uridine monophosphate and cytidine monophosphate. This enzyme does not phosphorylate deoxyribonucleosides or purine ribonucleosides. Of note, UCK1 is able to use either ATP or GTP as a phosphate donor. UCK1 also possesses the ability to phosphorylate a number of cytidine and uridine nucleoside analogs such as 6-azauridine, 5-fluorouridine, 4-thiouridine, 5-bromouridine, N(4)-acetylcytidine, N(4)-benzoylcytidine, 5-fluorocytidine, 2-thiocytidine, 5-methylcytidine, and N(4)-anisoylcytidine.

Synonyms: CMK, UMK, CMPK, UMPK, UMP-CMPK, RP11-511I2.1, Cmpk, UMP-CMPk, 0610011D08Rik, RP23-295C20.4, MGC112719, RGD1310116, cmpk1, cmpk, MGC64474, fb52d08, fk47a07, zgc:56452, wu:fb52d08, wu:fk47a07, CMPK1, MGC137127, LOC733534, DEZ, ChemR23, CHEMERINR, MGC126105, MGC126106, V28, CCRL1, GPR13, CMKDR1, GPRV28, CMKBRL1, PPR1, CCBP2, CCR10, CCR11, VSHK1, CKR-11, CCX-CKR, CC-CKR-11, Cmkbrl1, CCX-CKR1, A630091E18Rik

Application Details

Application Notes: The suggested dilution will be: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100 Flow cytometry

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

The anti-UCK Pab (ABIN392731) is used in Western blot to detect UCK in HepG2 cell lysate (Lane 1) and mouse cerebellum tissue lysate (Lane 2).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Hu, Han, Song et al.: "Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 17, pp. 9543-8, 2000 (PubMed).

Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).