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SAD1 (BRSK1) (N-Term) antibody
Western Blotting (WB), ELISA
|2 references available|
|Quantity||0.1 mg (0.25 mg/ml)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||This SAD1 (BRSK1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human BRSK1.|
|Description||Other names: BR serine/threonine-protein kinase 1, SAD1 kinase, SAD1A , BRSK1|
|Characteristics||Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This SAD1 (BRSK1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human BRSK1.|
|Molecular Weight||85086 DA|
Background: BRSK1 may be involved as a checkpoint kinase in the regulation of G2/M arrest in response to UV- or methyl methane sulfonate (MMS)-induced, but not IR-induced, DNA damage. This protein phosphorylates WEE1 and CDC25B in vitro and CDC25C in vitro and in vivo. BRSK1 is partitioned between cytoplasmic and nuclear locations in the absence of DNA damage, but translocates to the nucleus in response to Uv- or MMS-induced DNA damage. BRSK1 shares significant homology with the fission yeast Cdr2, a mitosis-regulatory kinase, and Caenorhabditis elegans SAD1, a neuronal cell polarity regulator. The BRSK1 transcript is expressed ubiquitously with the highest levels of expression in brain and testis.
|Application Notes||The suggested dilution will be: ELISA~~1:1,000 Western blotting~~1:100~500|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Phospho-specific antibodies, Cell Signaling, Protein Modifications, Neurology, Cell Structure|
|Restrictions||For Research Use only|
|Western blot analysis of anti-KIAA181 Pab (ABIN392801) in mouse liver tissue lysate (35ug/lane). KIAA181(arrow) was detected using the purified Pab (1:60 dilution).|
Lizcano, Göransson, Toth et al.: "LKB1 is a master kinase that activates 13 kinases of the AMPK subfamily, including MARK/PAR-1." in: The EMBO journal, Vol. 23, Issue 4, pp. 833-43, 2004 (PubMed).
Lu, Niida, Nakanishi: "Human SAD1 kinase is involved in UV-induced DNA damage checkpoint function." in: The Journal of biological chemistry, Vol. 279, Issue 30, pp. 31164-70, 2004 (PubMed).